CLONALE MICROPROPOGATION AND SANITATION OF PRUNUS DOMESTICA FROM THE PLUM POX POTYVIRUS (PPV)
نویسندگان
چکیده
منابع مشابه
Transcriptomic Analysis of Prunus domestica Undergoing Hypersensitive Response to Plum Pox Virus Infection
Plum pox virus (PPV) infects Prunus trees around the globe, posing serious fruit production problems and causing severe economic losses. One variety of Prunus domestica, named 'Jojo', develops a hypersensitive response to viral infection. Here we compared infected and non-infected samples using next-generation RNA sequencing to characterize the genetic complexity of the viral population in infe...
متن کاملTotal RNA extraction method and Prunus species influence the detection of Plum pox potyvirus by real-time RT-PCR
Successful total RNA (totRNA) extraction is a prerequisite for a successful real-time PCR. In the present work we compared one manual and one automated totRNA extraction method for detection of Plum pox potyvirus (PPV) in leaves of different Prunus sp. using developed real-time RT-PCR assay. Advantages and disadvantages of compared methods are described in the view of sensitivity, reproducibili...
متن کاملPotential Use of Transgenic Plums Resistant to Plum Pox Virus Field Infection
Research to date indicates that post-transcriptional gene silencing (PTGS) is an effective strategy for the development of stable high-level resistance to Plum pox virus in plum. Field tests in Poland, Romania, and Spain show that after 5–6 years of natural aphid vectored inoculation, trees of the PTGS clone C5 remain virus-free. The effectiveness of transgene-based PTGS for imparting potyvirus...
متن کاملSilencing in Genetically Engineered Prunus domestica Provides Durable and Safe Resistance to Plum pox virus (Sharka Disease)
Originally identified in Bulgaria in 1915, Plum pox virus (PPV) is the most damaging virus of stone fruit trees, including apricot, plum, peach and cherry. PPV steadily spread throughout Europe over the years since its discovery and at the turn of the century (1999-2000) it reached North America (USA and Canada). While many strategies to control the spread of PPV have been undertaken over the d...
متن کاملCloning of the complete infectious cDNA of the plum pox virus strain PPV-Rec.
UNLABELLED Plum pox virus (PPV) is the causal agent of Sharka, considered to be the most detrimental viral disease of Prunus spp. worldwide. So far, several PPV strains have been recognized, three of them (PPV-D, PPV-M, and PPV-Rec) having shown serious economic impact in the European area. Infectious cDNA clones of plant RNA viruses are excellent tools for functional studies of viral genomes. ...
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ژورنال
عنوان ژورنال: Polythematic Online Scientific Journal of Kuban State Agrarian University
سال: 2018
ISSN: 1990-4665
DOI: 10.21515/1990-4665-143-013